Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet all too often, nuances that are responsible for an experiment’s success are not captured in the primary literature, but exist only as part of a lab’s oral tradition. The aim of each Current Protocols title is to provide the clearest, most detailed and reliable step-by-step instructions for all protocols relevant to a given subject area. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols in Chemical Biology to constantly evolve to keep pace with the newest discoveries and developments.
Current Protocols in Chemical Biology provides clear step-by-step instructions for validated protocols as well as enlightening overviews for researchers who need specialized chemical techniques and tools for their studies of biology and drug design. Topics include:
synthesis, derivatization, handling, and detection;
laboratory automation and robotics;
high-throughput screening (HTS) methods;
modification and use of proteins, nucleic acids, carbohydrates and lipids to study biological systems.
The target audience for Current Protocols in Chemical Biology includes anyone interested in conducting research in biology and drug design using chemical techniques. Sufficient detail is provided to permit duplication of the protocols in any laboratory, whatever the disciplinary background and level of sophistication.